Brandywine Zoo is a small 12-acre (4.9 ha) zoo that opened in 1905 in Brandywine Park in Wilmington, Delaware, USA. It consists of the main zoo as well as a separate Exotic Animal House for small animals from hotter climates. It is located on the banks of the Brandywine River, and is the only zoo in Delaware. The zoo is managed by the Delaware Division of Parks and Recreation and supported by the Delaware Zoological Society, and is open daily.

The Brandywine Zoo has been a member of the Association of Zoos and Aquariums (AZA) since 1981.

In 2007, a Siberian tiger named Sergei died, leaving only Ashley the Siberian tiger in the habitat. On July 31, 2010, Ashley was humanely euthanized after 15 years in the Brandywine Zoo. She was born in 1990 and traveled from the St. Louis Zoo to the Brandywine when she was five years old. She lived to be 20 years old whereas tigers in the wild generally live to 10–12 years. The 20-year old tiger suffered complications from a brief illness and was unable to be saved. “Ashley had been very ill, and we were unable to save her after her condition rapidly deteriorated.” said Brandywine Zoo Director Nancy Falasco. “Ashley came to the Brandywine Zoo when she was five years of age,” Falasco said. She was housed with an older tiger named Kublai Khan. After Khan’s death years later, she was paired with Sergei, a hand-reared tiger that preferred to interact with people more than with other tigers. Their relationship was long but fraught with growls and the occasional fight. Through the help of the Delaware Zoological Society an additional enclosure was built for Ashley to reside in and to keep the tigers separated. Their relationship persisted with a protective fence between them. Sergei died in June 2007. In 2011, the Brandywine Zoo acquired an Amur tiger named Zhanna from the St. Louis Zoo.