U.S. Route 41, also U.S. Highway 41 (US 41), is a north–south United States Highway that runs from Miami, Florida to the Upper Peninsula of Michigan. Until 1949, the part in southern Florida, from Naples to Miami, was US 94, which presently has the hidden designation of State Road 90 (SR 90) in addition to its signed number.

The highway's northern terminus is east of Copper Harbor, Michigan, at a modest cul-de-sac near Fort Wilkins Historic State Park at the tip of the Keweenaw Peninsula in the state's Upper Peninsula. Its southern terminus is in the Brickell neighborhood of Downtown Miami at an intersection with Brickell Avenue (US 1). It closely parallels Interstate 75 (I-75) from Naples, Florida, all the way through Georgia to Chattanooga, Tennessee.