Shaw Prize in Life Science and Medicine 2004 Laureate

 

Stanley Norman Cohen

Stanley N Cohen was born in Perth Amboy, New Jersey, United States，February 17，1935 . Cohen was a graduate of Rutgers University, He received his MD from the University of Pennsylvania 1960. As an American geneticist. he is a Professor of Genetics and of Medicine, and Principal Investigator of Stanley N Cohen Lab at Stanford University.

It was there that he began to explore the field of bacterial plasmids. He wanted to understand how the genes of plasmids could make bacteria resistant to antibiotics. Cohen's investigations in 1972, combined with those of Paul Berg and Herbert Boyer, led to the development of methods to combine and transplant genes. This discovery signaled the birth of genetic engineering and earned Cohen the National Medal of Science in 1988. He also co-authored (with Royston C. Clowes, Roy Curtiss III, Naomi Datta, Stanley Falkow and Richard Novick) a proposal for uniform nomenclature for bacterial plasmids.

Today, Cohen is a professor of genetics and medicine at Stanford, where he works on a variety of scientific problems including cell growth and development.

Experiment
Stanley Cohen, Paul Berg and Herbert Boyer made what would be one of the first genetic engineering experiments, in 1973. They demonstrated that the gene for frog ribosomal RNA could be transferred into bacterial cells and expressed by them. First they developed a chemical cell transformation method for Escherichia coli, then they constructed a plasmid, which would be the vector, called pSC101. This plasmid contained a single site for the restriction enzyme EcoRI and a gene for tetracycline resistance. The restriction enzyme EcoRI was used to cut the frog DNA into small segments. Next, the frog DNA fragments were combined with the plasmid, which had also been cleaved with EcoRI.

The sticky ends of the DNA segments aligned themselves and were afterwards joined together using DNA ligase. The plasmids were then transferred into a strain of E. coli and plated onto a growth medium containing tetracycline. The cells that incorporated the plasmid carrying the tetracycline resistance gene grew and formed a colony of bacteria.

Some of these colonies consisted of cells that carried the frog ribosomal RNA gene. The scientists then tested the colonies that formed after growth for the presence of frog ribosomal DNA. (Thieman, W.J and Palladino, M.A., Introduction to Biotechnology, Pearson Education, Benjamin Cummings, 2024. page 55)

In 1961, S.N. Cohen married Joanna Lucy Wolter, whom he had met while he was a medical student. They have two children, Anne and Geoffrey.

Awards
1980 Albert Lasker Award for Basic Medical Research.
1988 National Medal of Science from President Reagan.
1989 National Medal of Technology.
2004 Albany Medical Center Prize (shared with Herbert Boyer)
2004 Shaw Prize in Life Science and Medicine.
2009 Double Helix Medal

Stanley Norman Cohen's genetic engineering laboratory, 1973
- National Museum of American History.

The Shaw Prize in Life Science and Medicine 2004 - Two Prizes

Prize One Laureates
Half jointly to Stanley N Cohen & Herbert W Boyer for their discoveries on DNA cloning and genetic engineering
Half to Yuet-Wai Kan for his discoveries on DNA polymorphism and its influence on human genetics.
 
Prize Two Laureate Richard Doll for his contribution to modern cancer epidemiology.